Antifungal, Antioxidant (Peroxynitrite scavenging, Hydroxyl radical scavenging and Nitric oxide radical scavenging) and Screening of Bioactive Chemical Compounds of Colocynth (Citrullus colocynthis) Using GCMS Technique

Abstract

Background: Inflammation of the joints, acne, hepatitis, urticarial infections, bowel irregularities, constipation, and bowel disturbance are some of the conditions that citrullus colocynthis helps alleviate. The glycosides and phenolic acids found in Citrullus colocynthis are extremely diverse in composition. Consequently, the purpose of this research was to examine the antifungal effects of Citrullus colocynthis fruit extract and to screen for bioactive components using GCMS.

Materials and Methods: The local plant parts that constitute the Citrullus colocynthis fruit and other plants were provided by provincial market of Babylon. The remaining plants were then sent for washing and separation from foreign bodies to the College of Science, the University of Babylon, to be studied intimately in the specialized Botanical laboratory. I guess these were some baked beans. “Everything here is completely smooth.” With counting the constants, we evaluated different components of spectra, and then the percentage relative peak area allowed us to determine all. These compounds were tentatively identified by comparing their respective kinetic retention times and the mass spectrum of the data obtained by GC-MS system, this system was purchased as the NIST and Wiley libraries. The diameter of the inhibition zone which is described in millimeters (mm) is the indicator of the antifungal activity of the fruit extracts.

Results : The secondary metabolites detected by GC-Mass technology are: 9-Octadecenoic acid, trans-Zeaxanthin, tetraneurin-A, Lycoxanthin, N-(4-Hydroxyphenyl-d4)retinamide, 1-tert-Butyl-4,4-diphenylpiperidine, Thiocarbamoylthioacetic acid, Lucenin-2, 4-(2,3-Diphenylcyclopropyl)phenol, 5-Aminoisothiazol-3-yl)methanol, 4,8,12,15,19,21-tetracosahexaenoic acid,  and Ethyl trans-2-phenylcyclopropanecarboxylate. Antifungal activity of secondary metabolites of Citrullus colocynthis: Bioactivity of the fruit extract (Ethyl acetate, and Ethanol) of Citrullus colocynthis and standard antibiotics against six fungi and yeast.  Alternaria alternaria (14.09 ± 0.28 and 17.61 ± 0.32), Aspergillus flavus  (22.30 ± 0.45 and 23.09 ± 0.47), Trichophyton rubrum (16.00 ± 0.49 and 20.74 ± 0.41), Fusarium oxyporum (24.08 ± 0.48 and 15.11 ± 0.49), Cladosporium herbarum (14.79 ± 0.28 and 19.00 ± 0.36), Cladosporium herbarum (6.10 ± 0.02 and 7.05 ± 0.04), Candida  albicans (21.00 ± 0.43 and 15.11 ± 0.49), and standard antibiotics (23.31 ± 0.48 and 27.09 ± 0.51) for Voriconazole (VCZ) and Amphotericin B (AmB) respectively.  Citrullus colocynthis metabolites was very highly-active against F. oxyporum (24.08 ± 0.48).

Conclusion: Based on the findings of this study, Citrullus colocynthis could be a great plant to look for if you're looking for active chemicals that can help with fungal infections.